We played last night, and it was a bit of a mistake- we had a guest player (the notorious Kristin Firth) and I should have tabled Undying for the night before we spent a long time doing character creation and set up, and then fumbled through learning the rules. Fine for longer term play, but not as much fun for her one night with us.
That said, we’ll be playing again and I’m reading through the rules again to do better. For your one shot, I would definitely recommend using the aggressive bang setup suggested in the book. Having everything consumed with fire, putting everything in chaos, is better than the slow, talky-talk murder mystery angle that I tried.
It’s worth noting that, this being diceless, the fiction is even more important than usual. Predators will have no trouble hunting and feeding, mechanically, so if you’re not narrating the process there’s not much meat to it.
In any case, I think you can do the R-map set up during play, but you’ll want to have an idea of who your NPCs are and what the likely hunting grounds are like ahead of time. You can work out the connections with the players.